RNA-Seq of Anabrus simplex: Adult female head - SRA

SRX11856153: RNA-Seq of Anabrus simplex: Adult female head
1 ILLUMINA (Illumina HiSeq 4000) run: 38.9M spots, 11.7G bases, 4.3Gb downloads

Design: Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. In order to select cDNA fragments of preferentially 250~300 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Subsequently, the library preparations were sequenced on an Illumina HiSeq4000 platform and paired-end reads were generated. After quality control and data filtering, these raw reads were pooled together to assemble a de novo transcriptome using Trinity with min_kmer_cov set to 2 by default and all other parameters set default. The reads were then filtered and clustered by Corset (v1.05) to remove redundancies and the longest transcripts per cluster were selected as unigenes.

Submitted by: Texas A&M University

Study: Anabrus simplex De Novo Transcriptome Assembly

PRJNA756613 • SRP333518 • All experiments • All runs

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The Mormon Cricket (Anabrus simplex Haldeman, 1852) is a species of flightless shield-backed katydid (Tettigoniidae) that occurs all over the Western Great plains of the United States. During favorable ecological conditions which promote high population densities, the Mormon Cricket will travel together in dense bands numbering in the millions, and can become a major pest in this aggregative state, consuming and damaging important agricultural crops and grazing fields. This project aims to sequence and assemble de novo transcriptomes of the Mormon Cricket based on individuals collected from an outbreak population in Idaho. Specifically three female adult individuals were selected to sequence tissue-specific transcriptomes from head, thorax, and abdomen.

Sample: Anabrus simplex head transcriptome from biological replicate 3

SAMN20893099 • SRS9867031 • All experiments • All runs

Organism: Anabrus simplex

Library:

Name: AsimH3

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: PAIRED

Runs: 1 run, 38.9M spots, 11.7G bases, 4.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR15558017	38,919,616	11.7G	4.3Gb	2021-08-21

ID:: 15773267

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